Homologs in group_262

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7 homologs were identified in 6 genomes with OrthoFinder.
The following table displays the locus tag of each homolog, the organism to which it belongs, the gene name and product.

Locus tag Identity Source Gene Product
FBDBKF_04725 FBDBKF_04725 80.9 Morganella morganii S1 pDC1 TPP-dependent 2-oxoacid decarboxylase, includes indolepyruvate decarboxylase
EHELCC_06015 EHELCC_06015 80.9 Morganella morganii S2 pDC1 TPP-dependent 2-oxoacid decarboxylase, includes indolepyruvate decarboxylase
NLDBIP_06335 NLDBIP_06335 80.9 Morganella morganii S4 pDC1 TPP-dependent 2-oxoacid decarboxylase, includes indolepyruvate decarboxylase
LHKJJB_03215 LHKJJB_03215 80.9 Morganella morganii S3 pDC1 TPP-dependent 2-oxoacid decarboxylase, includes indolepyruvate decarboxylase
HKOGLL_06690 HKOGLL_06690 80.9 Morganella morganii S5 pDC1 TPP-dependent 2-oxoacid decarboxylase, includes indolepyruvate decarboxylase
PMI_RS01075 PMI_RS01075 34.9 Proteus mirabilis HI4320 - thiamine pyrophosphate-binding protein
PMI_RS04150 PMI_RS04150 66.8 Proteus mirabilis HI4320 - thiamine pyrophosphate-binding protein

Distribution of the homologs in the orthogroup group_262

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Number of homologs in each genome (first column) and amino-acid identity of the closest homolog (second column).

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Phylogeny of the RefSeq best hits of group_262

Swissprot accession Eval Score ID (%) N gaps Alignment length Annot score Gene Description Organism
A0QBE6 1.11e-122 375 37 4 541 3 kdc Alpha-keto-acid decarboxylase Mycobacterium avium (strain 104)
Q742Q2 1.45e-122 374 37 4 541 3 kdc Alpha-keto-acid decarboxylase Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10)
P23234 7.83e-121 370 38 3 524 1 ipdC Indole-3-pyruvate decarboxylase Enterobacter cloacae
Q9CBD6 1.53e-117 362 37 5 545 3 kdc Alpha-keto-acid decarboxylase Mycobacterium leprae (strain TN)
P9WG37 5.95e-115 355 36 6 558 1 kdc Alpha-keto-acid decarboxylase Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
A5U0P1 5.95e-115 355 36 6 558 3 kdc Alpha-keto-acid decarboxylase Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
A1KGY5 5.95e-115 355 36 6 558 3 kdc Alpha-keto-acid decarboxylase Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Q7U140 5.95e-115 355 36 6 558 3 kdc Alpha-keto-acid decarboxylase Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
P9WG36 3.18e-114 353 36 6 558 3 kdc Alpha-keto-acid decarboxylase Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Q2UKV4 7.52e-114 352 39 10 516 3 pdcA Pyruvate decarboxylase Aspergillus oryzae (strain ATCC 42149 / RIB 40)
Q09737 1.52e-113 352 35 9 542 3 SPAC13A11.06 Putative pyruvate decarboxylase C13A11.06 Schizosaccharomyces pombe (strain 972 / ATCC 24843)
A0PL16 1.09e-112 349 36 4 541 3 kdc Alpha-keto-acid decarboxylase Mycobacterium ulcerans (strain Agy99)
P06169 1.05e-111 347 37 8 557 1 PDC1 Pyruvate decarboxylase isozyme 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Q4WXX9 4.89e-111 345 38 11 559 3 pdcA Pyruvate decarboxylase Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)
Q0CNV1 7.23e-110 342 39 8 516 3 pdcA Pyruvate decarboxylase Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Q6FJA3 7.44e-110 342 38 6 523 1 PDC1 Pyruvate decarboxylase Candida glabrata (strain ATCC 2001 / BCRC 20586 / JCM 3761 / NBRC 0622 / NRRL Y-65 / CBS 138)
P34734 8.2e-110 342 37 6 515 3 PDC Pyruvate decarboxylase Hanseniaspora uvarum
A0R480 7.01e-109 339 36 4 547 3 kdc Alpha-keto-acid decarboxylase Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
P83779 2.98e-108 338 36 11 554 1 PDC11 Pyruvate decarboxylase Candida albicans (strain SC5314 / ATCC MYA-2876)
P87208 4.18e-108 337 38 9 556 3 pdcA Pyruvate decarboxylase Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)
P26263 4.95e-108 337 36 8 528 1 PDC6 Pyruvate decarboxylase isozyme 3 Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
P16467 5.28e-108 337 37 6 528 1 PDC5 Pyruvate decarboxylase isozyme 2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
O42873 8.44e-107 334 35 10 539 3 SPAC3G9.11c Putative pyruvate decarboxylase C3G9.11c Schizosaccharomyces pombe (strain 972 / ATCC 24843)
P33149 2.01e-106 333 36 7 537 3 PDC1 Pyruvate decarboxylase Kluyveromyces marxianus
Q07471 4.24e-106 333 33 7 541 1 THI3 Thiamine metabolism regulatory protein THI3 Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Q12629 4.98e-105 329 36 7 537 1 PDC1 Pyruvate decarboxylase Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
P51844 3.28e-84 275 32 13 563 3 pdcA Pyruvate decarboxylase Aspergillus parasiticus
P06672 2.01e-78 260 32 6 563 1 pdc Pyruvate decarboxylase Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Q9M040 1.81e-77 258 32 9 548 2 PDC4 Pyruvate decarboxylase 4 Arabidopsis thaliana
Q0DHF6 1.12e-76 256 32 9 545 2 PDC1 Pyruvate decarboxylase 1 Oryza sativa subsp. japonica
Q9P7P6 1.29e-76 255 31 10 531 3 SPAC186.09 Probable pyruvate decarboxylase C186.09 Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Q92345 2.14e-76 254 34 10 526 1 SPAC1F8.07c Probable pyruvate decarboxylase C1F8.07c Schizosaccharomyces pombe (strain 972 / ATCC 24843)
A2Y5L9 4.4e-76 255 32 9 545 2 PDC1 Pyruvate decarboxylase 1 Oryza sativa subsp. indica
Q9FFT4 1.08e-75 254 32 10 548 2 PDC2 Pyruvate decarboxylase 2 Arabidopsis thaliana
P51846 5.22e-75 252 31 11 588 2 PDC2 Pyruvate decarboxylase 2 Nicotiana tabacum
A2XFI3 6.93e-75 252 31 10 542 2 PDC2 Pyruvate decarboxylase 2 Oryza sativa subsp. indica
Q9M039 5.47e-74 249 31 12 557 2 PDC3 Pyruvate decarboxylase 3 Arabidopsis thaliana
Q10MW3 6.11e-74 249 31 10 541 2 PDC2 Pyruvate decarboxylase 2 Oryza sativa subsp. japonica
P51850 1.73e-73 248 31 12 570 2 PDC1 Pyruvate decarboxylase 1 Pisum sativum
O82647 1.47e-72 246 30 9 548 2 PDC1 Pyruvate decarboxylase 1 Arabidopsis thaliana
P28516 2.99e-72 245 31 8 545 2 PDC1 Pyruvate decarboxylase 1 Zea mays
A2YQ76 2.66e-71 242 31 14 552 3 PDC3 Pyruvate decarboxylase 3 Oryza sativa subsp. indica
Q0D3D2 3.24e-71 241 31 14 557 2 PDC3 Pyruvate decarboxylase 3 Oryza sativa subsp. japonica
Q06408 2.32e-70 240 29 18 594 1 ARO10 Transaminated amino acid decarboxylase Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
P33287 5.3e-68 233 31 11 530 1 cfp Pyruvate decarboxylase Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
P51845 3.66e-52 186 32 8 422 2 PDC1 Pyruvate decarboxylase 1 (Fragment) Nicotiana tabacum
P51852 7.48e-42 161 27 16 556 1 ipdC Indole-3-pyruvate decarboxylase Azospirillum brasilense
P51851 1.45e-33 135 30 6 310 2 PDC2 Pyruvate decarboxylase 2 (Fragment) Pisum sativum
Q57725 7.12e-19 93 24 21 579 3 ilvB Probable acetolactate synthase large subunit Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Q05327 1.15e-18 87 31 3 176 2 PDC3 Pyruvate decarboxylase 3 (Fragment) Zea mays
P0A623 2.22e-18 92 22 14 546 3 ilvB Acetolactate synthase Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
P9WG41 2.22e-18 92 22 14 546 1 ilvB1 Acetolactate synthase large subunit IlvB1 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
P9WG40 2.22e-18 92 22 14 546 3 ilvB1 Acetolactate synthase large subunit IlvB1 Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
P0DUV9 9.76e-17 87 22 18 561 1 None 2-hydroxyacyl-CoA lyase Actinomycetospora chiangmaiensis (strain DSM 45062 / JCM 15998 / CCTCC AA 205017 / NBRC 104400 / YIM 0006)
Q59498 1.96e-16 86 22 16 561 3 ilvB Acetolactate synthase Mycobacterium avium
P45261 3.63e-16 85 22 19 560 3 ilvI Acetolactate synthase large subunit Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
P69684 1.44e-15 83 22 11 471 3 ilvB Acetolactate synthase large subunit Porphyra umbilicalis
P69683 1.44e-15 83 22 11 471 3 ilvB Acetolactate synthase large subunit Porphyra purpurea
Q1XDF6 3.2e-15 82 23 12 473 3 ilvB Acetolactate synthase large subunit Neopyropia yezoensis
O06335 3.87e-15 82 24 19 546 2 ilvB2 Putative acetolactate synthase large subunit IlvB2 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
O08353 1.71e-14 80 23 17 560 3 ilvB Probable acetolactate synthase large subunit Methanococcus aeolicus
P42463 2.41e-14 79 23 20 562 3 ilvB Acetolactate synthase large subunit Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534)
P40811 8.09e-14 77 22 13 489 3 ilvI Acetolactate synthase isozyme 3 large subunit Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
A3SR25 1.45e-13 77 22 24 596 1 xsc Sulfoacetaldehyde acetyltransferase Roseovarius nubinhibens (strain ATCC BAA-591 / DSM 15170 / ISM)
Q89AP7 1.7e-13 76 21 13 485 3 ilvI Acetolactate synthase large subunit Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Q9RQ65 4.51e-13 75 22 17 498 3 ilvI Acetolactate synthase large subunit Buchnera aphidicola subsp. Schlechtendalia chinensis
Q02137 4.85e-13 75 23 16 483 3 ilvB Acetolactate synthase large subunit Lactococcus lactis subsp. lactis (strain IL1403)
P00893 1.07e-12 74 22 12 483 1 ilvI Acetolactate synthase isozyme 3 large subunit Escherichia coli (strain K12)
P08142 1.44e-12 73 23 20 493 1 ilvB Acetolactate synthase isozyme 1 large subunit Escherichia coli (strain K12)
P0DP90 1.59e-12 73 23 17 479 1 ilvG Acetolactate synthase isozyme 2 large subunit Escherichia coli (strain K12)
O33112 1.8e-12 73 22 18 536 3 ilvB Acetolactate synthase Mycobacterium leprae (strain TN)
P57321 2.1e-12 73 21 14 482 3 ilvI Acetolactate synthase large subunit Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)
O85293 9.79e-12 71 22 16 493 3 ilvI Acetolactate synthase large subunit Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
P37251 3.23e-11 69 22 16 493 1 ilvB Acetolactate synthase large subunit Bacillus subtilis (strain 168)
A0A2I2F2I5 6.26e-11 68 21 23 582 3 cfoL Acetolactate synthase catalytic subunit, mitochondrial Aspergillus candidus
P27868 8.2e-11 68 22 17 494 3 ilvY Acetolactate synthase (Fragment) Arthrospira platensis
P07342 1.25e-10 67 22 22 573 1 ILV2 Acetolactate synthase catalytic subunit, mitochondrial Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Q7U5G1 3.05e-10 66 22 14 474 3 ilvB Acetolactate synthase large subunit Parasynechococcus marenigrum (strain WH8102)
Q6SSJ3 8.4e-10 65 21 24 568 3 ILV2 Acetolactate synthase, mitochondrial Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
P09342 1.28e-09 64 22 15 488 1 ALS Acetolactate synthase 1, chloroplastic Nicotiana tabacum SURA
Q84H41 1.32e-09 64 21 19 567 1 xsc Sulfoacetaldehyde acetyltransferase Alcaligenes xylosoxydans xylosoxydans
Q9QXE0 1.75e-09 63 21 19 551 1 Hacl1 2-hydroxyacyl-CoA lyase 1 Mus musculus
P07003 2.31e-09 63 22 17 564 1 poxB Pyruvate dehydrogenase [ubiquinone] Escherichia coli (strain K12)
Q5KPJ5 3.11e-09 63 21 24 568 3 ILV2 Acetolactate synthase, mitochondrial Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)
P09114 9.27e-09 62 22 16 483 1 ALS Acetolactate synthase 2, chloroplastic Nicotiana tabacum SURB
Q92UW6 2.15e-08 60 22 9 297 3 xsc Probable sulfoacetaldehyde acetyltransferase Rhizobium meliloti (strain 1021)
O19929 4.03e-08 59 22 8 336 3 ilvB Acetolactate synthase large subunit Cyanidium caldarium
P36620 4.54e-08 59 20 19 569 3 ilv1 Acetolactate synthase, mitochondrial Schizosaccharomyces pombe (strain 972 / ATCC 24843)
P17597 5.54e-08 59 22 17 485 1 ALS Acetolactate synthase, chloroplastic Arabidopsis thaliana
P27819 6.44e-08 59 22 16 478 3 None Acetolactate synthase 3, chloroplastic Brassica napus
P96591 7.24e-08 58 20 17 550 2 ydaP Putative thiamine pyrophosphate-containing protein YdaP Bacillus subtilis (strain 168)
Q41768 8.4e-08 58 21 14 481 3 ALS1 Acetolactate synthase 1, chloroplastic Zea mays
O78518 1.25e-07 58 24 9 294 3 ilvB Acetolactate synthase large subunit Guillardia theta
O78518 6.93e-05 49 36 0 55 3 ilvB Acetolactate synthase large subunit Guillardia theta
P27818 1.51e-07 58 22 16 478 3 None Acetolactate synthase 1, chloroplastic Brassica napus
Q04789 3.47e-07 56 23 20 481 2 alsS Acetolactate synthase Bacillus subtilis (strain 168)
Q0JMH0 9.31e-07 55 26 4 169 3 Os01g0505400 2-hydroxyacyl-CoA lyase Oryza sativa subsp. japonica
Q6NV04 1.71e-06 54 20 19 516 2 ilvbl 2-hydroxyacyl-CoA lyase 2 Danio rerio
Q84H44 2.8e-06 53 23 9 278 1 xsc Sulfoacetaldehyde acetyltransferase Castellaniella defragrans
Q9LF46 6.29e-06 52 25 5 172 1 HACL 2-hydroxyacyl-CoA lyase Arabidopsis thaliana
Q9LF46 0.000262 47 23 11 261 1 HACL 2-hydroxyacyl-CoA lyase Arabidopsis thaliana
Q9HUR2 3.75e-05 50 23 8 272 3 mdlC Benzoylformate decarboxylase Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
P20906 0.000162 48 20 6 269 1 mdlC Benzoylformate decarboxylase Pseudomonas putida
Q8CHM7 0.000399 47 23 8 256 1 Hacl1 2-hydroxyacyl-CoA lyase 1 Rattus norvegicus
Q9LCV9 0.00058 46 30 4 130 1 ceaS N(2)-(2-carboxyethyl)arginine synthase Streptomyces clavuligerus

  • Number of RefSeq hits:

General

Source Morganella psychrotolerans
Locus tag F4V73_RS09200
Feature type CDS
Gene -
Product thiamine pyrophosphate-binding protein
Location 1926899 - 1928548 (strand: 1)
Length 1650 (nucleotides) / 549 (amino acids)

Contig

Accession term accessions NZ_VXKB01000001 accessions NZ_VXKB01000000 Name: value, dtype: object
Length 2012992 nucleotides
Topology linear
Plasmid False

Orthology

Orthogroup group_262
Orthogroup size 8
N. genomes 7

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Genomic region

Domains

PF00205 Thiamine pyrophosphate enzyme, central domain
PF02775 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
PF02776 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain

COG entry Annotation(s)

ID Function(s) descr. Function(s) cat. Description
COG3961 Carbohydrate transport and metabolism (G)
Coenzyme transport and metabolism (H)
General function prediction only (R)		 GHR TPP-dependent 2-oxoacid decarboxylase, includes indolepyruvate decarboxylase

Kegg Ortholog Annotation(s)

KO Description Pathways Modules
K04103 indolepyruvate decarboxylase [EC:4.1.1.74] Tryptophan metabolism
Metabolic pathways		 -

Protein Sequence

MTKTVIEHVLGRLQDLGIHHVFGVAGDYAFPVEDAVCESKSMKWIGNCNELNAAYAADGYARINGMAALSTTFGVGELSAINGIAGSYAENLPVFHLVGMPASGVQKSGRLVHHTLGDGDFNLFYELGQRLTCAHAIMTPENCVQETERLIISALRERRPVYIGFPSDYAVLPVKTPETVTPQVLPVSNSASLSAAVAAISAKLAASKSTCILPGMLSARSGLIEDVRALISKTGLPYATMFMDKAIISESDPHYTGMYDGKLMNPQVREFVENCECILSVGAVVTDFNTGSFTAEIAPQKLISIMAGHVSVGAAVFHDVYMRDILPALIAALPHTDCPVPPATGLGLPVSDVSGEIIPQYLYPRFEQMFRNNDIIIAETGTVSMGLGFALLPEGAHFHNQTLWGSIGWATPAAFGAAVAAPDKRIILITGEGAHQLTAQEISQFDRAGLKPLIFVLNNDGYLIERLLCKDPEAVYNDLPQWRYAQLPEALGCDDWYCRRVTTCTELDNAIKEAESGDRAAYIEIITNRYAASELAEKLGESAASLYSF

Flanking regions ( +/- flanking 50bp)

AATGATTTGACGCTGATAAATCAGCACCGTATTTACCGGAGAAGAAGAAAATGACAAAAACAGTGATTGAGCATGTGCTGGGGCGTTTGCAGGATCTCGGTATCCACCATGTATTTGGTGTCGCCGGTGACTATGCATTCCCGGTTGAAGATGCCGTATGCGAAAGCAAATCCATGAAATGGATAGGAAATTGTAATGAACTGAACGCCGCCTACGCCGCCGATGGCTATGCACGCATCAACGGAATGGCGGCGCTTTCAACCACCTTCGGCGTCGGAGAATTAAGCGCCATAAACGGTATTGCCGGCTCTTATGCCGAAAATCTGCCGGTTTTTCATCTGGTTGGTATGCCGGCAAGTGGTGTGCAAAAAAGCGGGCGCCTGGTTCACCATACATTAGGTGACGGGGATTTTAACCTGTTTTATGAACTGGGGCAGCGCCTGACCTGCGCCCACGCAATAATGACACCGGAGAATTGTGTACAGGAAACAGAGCGGCTTATCATCAGCGCCCTGCGTGAGCGCCGCCCTGTCTATATCGGCTTTCCGTCTGATTACGCCGTATTGCCGGTTAAAACACCTGAAACTGTCACTCCGCAGGTGCTGCCGGTCAGTAACAGCGCCTCACTGTCTGCGGCTGTTGCCGCCATCAGCGCTAAGCTGGCGGCAAGCAAAAGCACCTGTATTTTACCCGGCATGTTATCTGCCCGATCCGGGTTAATTGAAGATGTCCGCGCACTCATCAGTAAAACAGGTCTGCCATACGCCACCATGTTTATGGATAAAGCGATTATCAGCGAATCCGATCCCCATTACACCGGCATGTATGACGGGAAACTGATGAATCCGCAGGTCAGGGAGTTTGTTGAAAATTGTGAATGTATCCTGAGTGTCGGTGCCGTTGTGACTGATTTCAATACCGGTAGTTTTACCGCAGAGATTGCCCCACAGAAACTCATCAGCATTATGGCGGGTCACGTCAGTGTCGGCGCAGCCGTTTTCCATGATGTGTATATGCGGGATATTCTCCCGGCGTTAATCGCCGCGTTACCCCACACAGATTGTCCTGTTCCGCCTGCAACCGGGCTTGGTTTGCCTGTGTCGGATGTATCCGGCGAGATAATACCGCAATATCTTTATCCGCGTTTTGAACAAATGTTCAGAAATAACGATATTATTATTGCTGAAACCGGAACCGTTTCAATGGGGCTGGGCTTTGCTTTGCTGCCGGAAGGGGCACATTTTCATAACCAAACGTTATGGGGTTCAATCGGCTGGGCAACACCGGCGGCATTCGGGGCTGCGGTTGCCGCACCGGACAAACGAATTATCCTGATAACCGGGGAAGGCGCTCATCAGCTGACCGCACAGGAGATAAGCCAGTTTGACCGCGCCGGGCTGAAACCTCTTATTTTTGTGCTGAATAATGATGGCTATCTGATTGAGCGGCTATTGTGCAAAGACCCGGAGGCTGTTTATAACGATTTGCCGCAATGGCGCTATGCACAATTGCCGGAAGCACTGGGGTGTGATGACTGGTATTGCCGCCGCGTCACCACCTGCACTGAACTGGATAACGCGATTAAAGAGGCAGAGTCCGGTGACCGCGCCGCTTATATTGAAATCATAACAAACCGGTATGCTGCCTCTGAACTGGCTGAAAAACTGGGGGAATCAGCGGCATCCCTGTATTCGTTTTAACGGAACCCGGCAGCGGGTTCTTTATTTCAGAACCCGTTTTCTTTACACAA