Homologs in group_28

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14 homologs were identified in 7 genomes with OrthoFinder.
The following table displays the locus tag of each homolog, the organism to which it belongs, the gene name and product.

Locus tag Identity Source Gene Product
FBDBKF_00525 FBDBKF_00525 71.6 Morganella morganii S1 flgL Flagellin and related hook-associated protein FlgL
EHELCC_01020 EHELCC_01020 71.6 Morganella morganii S2 flgL Flagellin and related hook-associated protein FlgL
EHELCC_01025 EHELCC_01025 100.0 Morganella morganii S2 - Flagellin
NLDBIP_02435 NLDBIP_02435 100.0 Morganella morganii S4 - Flagellin
NLDBIP_02440 NLDBIP_02440 71.6 Morganella morganii S4 flgL Flagellin and related hook-associated protein FlgL
LHKJJB_03950 LHKJJB_03950 100.0 Morganella morganii S3 - Flagellin
LHKJJB_03955 LHKJJB_03955 71.6 Morganella morganii S3 flgL Flagellin and related hook-associated protein FlgL
HKOGLL_03090 HKOGLL_03090 71.6 Morganella morganii S5 flgL Flagellin and related hook-associated protein FlgL
HKOGLL_03095 HKOGLL_03095 100.0 Morganella morganii S5 - Flagellin
F4V73_RS06530 F4V73_RS06530 70.1 Morganella psychrotolerans - FliC/FljB family flagellin
F4V73_RS06535 F4V73_RS06535 71.9 Morganella psychrotolerans - FliC/FljB family flagellin
F4V73_RS06540 F4V73_RS06540 71.9 Morganella psychrotolerans - FliC/FljB family flagellin
PMI_RS07910 PMI_RS07910 66.7 Proteus mirabilis HI4320 - FliC/FljB family flagellin
PMI_RS07915 PMI_RS07915 66.5 Proteus mirabilis HI4320 - FliC/FljB family flagellin

Distribution of the homologs in the orthogroup group_28

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Number of homologs in each genome (first column) and amino-acid identity of the closest homolog (second column).

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Phylogeny of the RefSeq best hits of group_28

Swissprot accession Eval Score ID (%) N gaps Alignment length Annot score Gene Description Organism
P13713 4.86e-128 374 58 5 361 3 fliC Flagellin Serratia marcescens
P42272 4.58e-123 361 61 8 370 1 fliC1 Flagellin 1 Proteus mirabilis
P42273 7.02e-123 361 59 8 379 2 fliC2 Flagellin 2 Proteus mirabilis
Q06064 1.02e-114 341 52 7 398 1 flaA Flagellin Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50)
Q56826 4.91e-107 319 57 6 358 3 fliC Flagellin Xenorhabdus nematophila
Q06983 1.4e-82 263 66 3 209 3 fliC Flagellin Salmonella senftenberg
Q06983 8.64e-31 125 72 0 84 3 fliC Flagellin Salmonella senftenberg
Q06972 1.6e-82 262 66 2 209 3 fliC Flagellin Salmonella enteritidis
Q06972 8.22e-31 125 72 0 84 3 fliC Flagellin Salmonella enteritidis
Q06969 1.65e-82 262 66 2 209 3 fliC Flagellin Salmonella budapest
Q06969 8.9e-31 125 72 0 84 3 fliC Flagellin Salmonella budapest
Q06981 1.76e-82 262 66 2 209 3 fliC Flagellin Salmonella moscow
Q06981 8.73e-31 125 72 0 84 3 fliC Flagellin Salmonella moscow
Q06971 1.78e-82 262 66 2 209 1 fliC Flagellin Salmonella dublin
Q06971 8.47e-31 125 72 0 84 1 fliC Flagellin Salmonella dublin
Q06982 1.98e-82 262 66 2 209 3 fliC Flagellin Salmonella rostock
Q06982 8.47e-31 125 72 0 84 3 fliC Flagellin Salmonella rostock
O52959 2.04e-82 262 66 2 209 3 fliC Phase 1 flagellin Salmonella naestved
O52959 8.81e-31 125 72 0 84 3 fliC Phase 1 flagellin Salmonella naestved
Q06970 2.09e-82 262 66 2 209 3 fliC Flagellin Salmonella derby
Q06970 9.54e-31 125 72 0 84 3 fliC Flagellin Salmonella derby
Q06974 2.14e-82 262 73 0 175 3 fliC Flagellin Salmonella oranienberg
Q06974 8.46e-31 125 72 0 84 3 fliC Flagellin Salmonella oranienberg
Q06968 3.18e-82 262 73 0 175 3 fliC Flagellin Salmonella berta
Q06968 9.06e-31 125 72 0 84 3 fliC Flagellin Salmonella berta
Q06973 3.42e-82 261 73 0 175 3 fliC Flagellin Salmonella montevideo
Q06973 8.47e-31 125 72 0 84 3 fliC Flagellin Salmonella montevideo
P06177 5.93e-80 256 68 1 189 3 fliC Flagellin Salmonella muenchen
P06177 1.18e-32 130 74 0 86 3 fliC Flagellin Salmonella muenchen
P06179 6.45e-80 255 56 5 257 1 fliC Flagellin Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
P06179 2.32e-32 129 73 0 86 1 fliC Flagellin Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
P52615 4.14e-79 253 56 3 254 3 fljB Phase 2 flagellin Salmonella abortus-equi
P52615 1.26e-32 130 74 0 86 3 fljB Phase 2 flagellin Salmonella abortus-equi
P06176 5.71e-79 253 72 0 170 3 fliC Flagellin Salmonella choleraesuis (strain SC-B67)
P06176 7.26e-33 131 74 0 86 3 fliC Flagellin Salmonella choleraesuis (strain SC-B67)
P06175 6.69e-79 253 72 0 170 3 fliC Flagellin Salmonella rubislaw
P06175 2.29e-32 129 73 0 86 3 fliC Flagellin Salmonella rubislaw
P06178 7.51e-79 253 63 2 213 3 fliC Flagellin Salmonella paratyphi A (strain ATCC 9150 / SARB42)
P06178 2.14e-32 129 75 0 85 3 fliC Flagellin Salmonella paratyphi A (strain ATCC 9150 / SARB42)
P52616 1.54e-78 252 70 0 175 1 fljB Phase 2 flagellin Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
P52616 1.8e-32 130 74 0 86 1 fljB Phase 2 flagellin Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
B7USU2 9.6e-76 246 68 0 175 1 fliC Flagellin Escherichia coli O127:H6 (strain E2348/69 / EPEC)
B7USU2 4.53e-23 103 66 0 84 1 fliC Flagellin Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Q08860 1.52e-75 246 68 0 175 3 fliC Flagellin Shigella flexneri
Q08860 1.55e-24 107 67 0 86 3 fliC Flagellin Shigella flexneri
P04949 9.03e-75 242 66 0 175 1 fliC Flagellin Escherichia coli (strain K12)
P04949 1.57e-24 107 48 3 160 1 fliC Flagellin Escherichia coli (strain K12)
P21184 6.2e-73 234 40 9 403 1 fliC A-type flagellin Pseudomonas aeruginosa
P0C6C7 1.01e-69 226 40 10 394 3 flaE Flagellin E Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
A5F6A9 1.01e-69 226 40 10 394 3 flaE Flagellin E Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Q56572 2.19e-69 224 41 13 384 1 flaB Flagellin B Vibrio anguillarum
O87081 2.41e-69 224 39 8 387 3 flaF Polar flagellin F Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Q60246 6.76e-69 223 40 8 390 3 flaA Flagellin A Vibrio anguillarum
P0C6C6 1.14e-67 220 40 11 394 1 flaD Flagellin D Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
A5F6C5 1.14e-67 220 40 11 394 3 flaD Flagellin D Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Q56712 3.85e-67 219 40 9 387 3 flaC Polar flagellin C Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Q56703 5.43e-66 216 39 11 392 3 flaA Polar flagellin A Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
P0C6C5 6.48e-66 216 39 10 391 3 flaC Flagellin C Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
A5F671 6.48e-66 216 39 10 391 3 flaC Flagellin C Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
P0C6C3 3.34e-65 214 39 8 382 3 flaA Flagellin A Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
A5F672 3.34e-65 214 39 8 382 3 flaA Flagellin A Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Q56570 4.37e-65 213 37 11 395 3 flaE Probable flagellin E Vibrio anguillarum
Q56574 5.3e-64 211 39 10 391 1 flaC Flagellin C Vibrio anguillarum
Q56702 2.28e-62 206 38 11 400 3 flaB Polar flagellin B/D Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
P0C6C4 2.28e-62 206 39 7 393 3 flaB Flagellin B Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
A5F6C4 2.28e-62 206 39 7 393 3 flaB Flagellin B Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Q56571 1.19e-59 199 39 9 383 1 flaD Flagellin D Vibrio anguillarum
P80583 1.39e-52 181 36 12 387 1 fla Flagellin (Fragment) Clostridium tyrobutyricum
Q05203 4.06e-47 164 33 7 356 1 hag Flagellin Halalkalibacterium halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Q9ZBA2 6.99e-46 163 35 11 384 3 flaE Polar flagellin E Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
P53606 2.44e-43 159 52 2 171 3 fla Flagellin Legionella micdadei
P53606 1.13e-25 110 65 0 84 3 fla Flagellin Legionella micdadei
P11089 3.02e-43 155 33 9 358 1 fla Flagellar filament 41 kDa core protein Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
P72151 2.1e-40 151 49 2 171 1 fliC B-type flagellin Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
P72151 4.3e-23 103 59 0 84 1 fliC B-type flagellin Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q03473 3.53e-35 133 44 1 164 3 lafA Lateral flagellin Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Q03473 3.33e-19 89 43 2 116 3 lafA Lateral flagellin Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
P02968 1.33e-31 124 41 4 199 1 hag Flagellin Bacillus subtilis (strain 168)
P21989 8.56e-29 115 39 2 180 1 None Flagellar filament 33 kDa core protein Treponema phagedenis
P21991 1.18e-28 115 38 2 180 1 flaB2 Flagellin FlaB2 Treponema pallidum (strain Nichols)
P21991 8.01e-19 88 52 0 84 1 flaB2 Flagellin FlaB2 Treponema pallidum (strain Nichols)
P21990 1.65e-28 115 36 1 180 1 flaB1 Flagellin FlaB1 Treponema pallidum (strain Nichols)
P21990 6.07e-16 80 43 1 101 1 flaB1 Flagellin FlaB1 Treponema pallidum (strain Nichols)
Q9KWX0 5.93e-26 108 43 2 139 1 flaB2 Flagellar filament 31.3 kDa core protein Treponema maltophilum
Q9KWX0 4.51e-15 78 35 3 143 1 flaB2 Flagellar filament 31.3 kDa core protein Treponema maltophilum
P21992 7.78e-25 105 40 4 159 1 flaB3 Flagellin FlaB3 Treponema pallidum (strain Nichols)
P21992 1.74e-14 76 45 0 84 1 flaB3 Flagellin FlaB3 Treponema pallidum (strain Nichols)
Q72R59 2.15e-24 103 38 2 155 3 flaB Flagellar filament 35 kDa core protein Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
Q72R59 1.5e-13 73 45 0 83 3 flaB Flagellar filament 35 kDa core protein Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
Q9KWW9 2.35e-24 103 38 1 156 1 flaB3 Flagellar filament 30.7 kDa core protein Treponema maltophilum
O51941 3.36e-24 103 38 2 155 1 flaB Flagellar filament 35 kDa core protein Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
O51941 1.47e-13 73 45 0 83 1 flaB Flagellar filament 35 kDa core protein Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
P80160 8.39e-24 102 40 2 154 1 flaB2 Flagellar filament core protein flaB2 Brachyspira hyodysenteriae
P80160 8.81e-11 65 43 1 94 1 flaB2 Flagellar filament core protein flaB2 Brachyspira hyodysenteriae
Q92DW3 3.61e-23 100 36 1 161 3 flaA Flagellin Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262)
Q92DW3 1.62e-11 67 34 2 128 3 flaA Flagellin Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262)
Q02551 3.65e-23 100 36 1 161 3 flaA Flagellin Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Q02551 3.03e-11 67 34 2 128 3 flaA Flagellin Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
O34366 3.75e-20 89 52 0 90 1 yvzB Putative flagellin YvzB Bacillus subtilis (strain 168)
P27053 2.52e-17 86 34 1 175 3 flaA Flagellin A Campylobacter coli
P27053 2.52e-11 68 42 0 83 3 flaA Flagellin A Campylobacter coli
P56963 4.25e-17 85 35 2 176 1 flaA Flagellin A Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
P56963 2.35e-12 71 44 0 83 1 flaA Flagellin A Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
P22251 1.25e-16 84 34 1 175 1 flaA Flagellin A Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
P22251 5.98e-12 70 43 0 83 1 flaA Flagellin A Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
Q46113 1.93e-16 84 35 2 176 3 flaA Flagellin A Campylobacter jejuni
Q46113 2.4e-12 71 44 0 83 3 flaA Flagellin A Campylobacter jejuni
P46210 2.33e-16 83 28 6 263 3 flaA Flagellin Aquifex pyrophilus
P46210 2.49e-06 52 38 0 81 3 flaA Flagellin Aquifex pyrophilus
P18245 3.62e-16 83 34 1 175 3 flaB Flagellin B Campylobacter coli
P18245 3.16e-11 68 43 0 83 3 flaB Flagellin B Campylobacter coli
O67803 4.43e-16 82 32 2 171 1 flaA Flagellin Aquifex aeolicus (strain VF5)
O67803 6.46e-09 60 30 5 197 1 flaA Flagellin Aquifex aeolicus (strain VF5)
P56964 4.71e-16 82 36 2 176 3 flaB Flagellin B Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
P56964 2.83e-11 68 43 0 83 3 flaB Flagellin B Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
P22252 5.96e-16 82 34 1 175 1 flaB Flagellin B Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
P22252 5.46e-12 70 43 0 83 1 flaB Flagellin B Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
Q46114 7.15e-16 82 36 2 176 3 flaB Flagellin B Campylobacter jejuni
Q46114 1.03e-11 69 43 0 83 3 flaB Flagellin B Campylobacter jejuni
Q07910 1.27e-14 78 30 1 182 3 flaB Flagellin B Helicobacter mustelae
Q07910 3.06e-12 71 46 0 84 3 flaB Flagellin B Helicobacter mustelae
P0A0S1 5.79e-13 73 45 0 82 1 flaA Flagellin A Helicobacter pylori (strain ATCC 700392 / 26695)
P0A0S1 5.24e-12 70 31 1 170 1 flaA Flagellin A Helicobacter pylori (strain ATCC 700392 / 26695)
P0A0S2 5.79e-13 73 45 0 82 3 flaA Flagellin A Helicobacter pylori (strain J99 / ATCC 700824)
P0A0S2 5.24e-12 70 31 1 170 3 flaA Flagellin A Helicobacter pylori (strain J99 / ATCC 700824)
Q9XB38 9.93e-13 72 46 0 82 3 flaA Flagellin A Helicobacter felis (strain ATCC 49179 / CCUG 28539 / NCTC 12436 / CS1)
Q9XB38 1.16e-12 72 35 7 181 3 flaA Flagellin A Helicobacter felis (strain ATCC 49179 / CCUG 28539 / NCTC 12436 / CS1)
P50612 1.32e-11 68 32 2 166 1 flaA Flagellin A Helicobacter mustelae
P50612 3.22e-06 52 43 0 62 1 flaA Flagellin A Helicobacter mustelae
Q9ZMV8 6.43e-11 67 44 0 83 3 flaB Flagellin B Helicobacter pylori (strain J99 / ATCC 700824)
Q9ZMV8 2.4e-09 62 32 4 186 3 flaB Flagellin B Helicobacter pylori (strain J99 / ATCC 700824)
Q07911 6.85e-11 67 44 0 83 1 flaB Flagellin B Helicobacter pylori (strain ATCC 700392 / 26695)
Q07911 2.56e-09 62 32 4 186 1 flaB Flagellin B Helicobacter pylori (strain ATCC 700392 / 26695)
P18913 2.55e-06 52 25 2 167 1 fljK Flagellin FljK Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15)
P02969 1.03e-05 50 26 7 238 3 fljJ Flagellin FljJ Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15)
O52531 0.000109 47 25 1 143 3 fljO Flagellin FljO Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15)
P18914 0.000445 45 22 2 171 3 fljL Flagellin FljL Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15)
O52529 0.001 44 24 1 143 3 fljM Flagellin FljM Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15)

  • Number of RefSeq hits:

General

Source Morganella morganii S1
Locus tag FBDBKF_00520
Feature type CDS
Gene -
Product Flagellin
Location 87351 - 88421 (strand: -1)
Length 1071 (nucleotides) / 356 (amino acids)

Contig

Accession contig_1
Length 309072 nucleotides
Topology linear
Plasmid False

Orthology

Orthogroup group_28
Orthogroup size 15
N. genomes 7

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Genomic region

Domains

PF00669 Bacterial flagellin N-terminal helical region
PF00700 Bacterial flagellin C-terminal helical region

COG entry Annotation(s)

ID Function(s) descr. Function(s) cat. Description
COG1344 Cell motility (N) N Flagellin and related hook-associated protein FlgL

Kegg Ortholog Annotation(s)

Protein Sequence

MAQVINTNVLSLVTQNNLNRSQGVLGTAIERLSSGARINSAKDDAAGQAIANRFTANVKGLAQASRNANDGISIAQTAEGAVNEINDNIQRIRELTVQAKNGTNSKSDIGSIQEEVNERLSEIKRISEQTQFNGIKVLEQGTKMNFQVGANDGEVIELDMQKIDLAKLGLKDFELPTYTALKSDTKLDATAEKAEAAKVDATFTTVDLKGHELFETATGTIVAKKGADYFEVKTFEKDGTTATQGNLVVGDKLTDIKSKADADKVNDATTKKLDEALKTVDSYRSKLGAFQNRMQSTINNLNNSVNNLSAARSRIQDADFATEVSNMSRGQILQQAGTAVLAQANQVPQGVLSLLR

Flanking regions ( +/- flanking 50bp)

GCCAAACAGATAAACGCTGTTTATTATTTTCAATAACAAGGACATATATCATGGCACAGGTTATCAATACTAACGTTCTGTCTCTGGTTACTCAGAACAACTTAAACCGTTCACAGGGCGTATTAGGCACTGCAATTGAGCGTCTGTCTTCAGGTGCCCGCATCAACAGCGCAAAAGATGACGCTGCAGGCCAGGCAATCGCCAACCGTTTCACCGCTAACGTAAAAGGCCTGGCACAGGCTTCCCGTAACGCTAACGACGGTATCTCTATCGCACAGACCGCTGAAGGTGCTGTTAACGAAATCAACGACAACATCCAGCGCATCCGTGAACTGACTGTTCAGGCGAAAAACGGCACTAACTCCAAGAGCGATATCGGTTCTATCCAGGAAGAAGTGAACGAGCGCCTGTCAGAAATCAAACGTATTTCTGAACAAACTCAGTTCAACGGCATTAAAGTGCTGGAGCAGGGCACTAAAATGAACTTCCAGGTTGGTGCAAACGACGGTGAAGTCATTGAACTGGATATGCAGAAAATTGATCTGGCTAAACTGGGTCTGAAAGATTTCGAACTGCCGACTTATACTGCTCTGAAATCAGATACTAAGCTTGATGCAACAGCAGAAAAAGCAGAGGCTGCTAAAGTAGATGCAACATTTACGACTGTTGATCTGAAAGGCCACGAACTGTTTGAAACAGCAACCGGTACTATCGTTGCTAAGAAAGGTGCGGATTATTTTGAAGTGAAAACCTTCGAAAAAGACGGAACTACAGCGACACAAGGTAACCTGGTCGTTGGTGATAAACTGACTGATATCAAGTCCAAAGCAGATGCTGACAAAGTCAACGATGCAACCACCAAAAAACTGGACGAAGCACTGAAAACTGTTGACTCATACCGTTCAAAACTGGGTGCATTCCAGAACCGTATGCAGTCTACCATCAACAACCTGAACAACTCTGTTAACAACCTGAGCGCAGCACGCAGCCGTATCCAGGACGCTGACTTCGCAACAGAAGTGTCTAACATGAGCCGTGGTCAGATCCTGCAGCAGGCGGGTACCGCTGTTCTGGCACAGGCAAACCAGGTTCCTCAGGGTGTTCTGAGCCTGTTACGTTAATTTACGTAGTTGCTTTAGCGTGTTTAAAAGGTCAGAATATTCTGACCTTT