Homologs in group_2454

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6 homologs were identified in 1 genome with OrthoFinder.
The following table displays the locus tag of each homolog, the organism to which it belongs, the gene name and product.

Locus tag Identity Source Gene Product
PMI_RS05695 PMI_RS05695 39.8 Proteus mirabilis HI4320 - methyl-accepting chemotaxis protein
PMI_RS08130 PMI_RS08130 33.8 Proteus mirabilis HI4320 - methyl-accepting chemotaxis protein
PMI_RS08135 PMI_RS08135 33.4 Proteus mirabilis HI4320 - methyl-accepting chemotaxis protein
PMI_RS11780 PMI_RS11780 58.5 Proteus mirabilis HI4320 - methyl-accepting chemotaxis protein
PMI_RS13870 PMI_RS13870 31.8 Proteus mirabilis HI4320 - methyl-accepting chemotaxis protein
PMI_RS13875 PMI_RS13875 31.8 Proteus mirabilis HI4320 - methyl-accepting chemotaxis protein

Distribution of the homologs in the orthogroup group_2454

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Number of homologs in each genome (first column) and amino-acid identity of the closest homolog (second column).

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Phylogeny of the RefSeq best hits of group_2454

Swissprot accession Eval Score ID (%) N gaps Alignment length Annot score Gene Description Organism
P21823 9.34e-79 259 41 0 353 3 tas Methyl-accepting chemotaxis aspartate transducer Klebsiella aerogenes (strain ATCC 13048 / DSM 30053 / CCUG 1429 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006 / CDC 819-56)
Q02755 1.84e-76 253 39 1 396 3 tcp Methyl-accepting chemotaxis citrate transducer Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
P05704 6.82e-73 243 42 1 333 1 trg Methyl-accepting chemotaxis protein III Escherichia coli (strain K12)
P07018 1.48e-71 239 43 1 321 1 tap Methyl-accepting chemotaxis protein IV Escherichia coli (strain K12)
P07017 1.66e-71 240 46 1 307 1 tar Methyl-accepting chemotaxis protein II Escherichia coli (strain K12)
P21822 2.57e-70 237 42 0 326 3 tse Methyl-accepting chemotaxis serine transducer Klebsiella aerogenes (strain ATCC 13048 / DSM 30053 / CCUG 1429 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006 / CDC 819-56)
P02941 2.44e-69 234 47 1 299 1 tar Methyl-accepting chemotaxis protein II Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
P02942 6.6e-68 231 40 1 357 1 tsr Methyl-accepting chemotaxis protein I Escherichia coli (strain K12)
Q9I6V6 2.29e-59 211 44 0 267 1 mcpB Methyl-accepting chemotaxis protein McpB Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q00986 1.37e-53 194 37 0 296 3 mcpA Chemoreceptor McpA Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15)
P50466 5.53e-46 171 36 0 286 1 aer Aerotaxis receptor Escherichia coli (strain K12)
P55439 5.25e-40 157 34 0 300 3 NGR_a03800 Probable chemoreceptor y4fA Sinorhizobium fredii (strain NBRC 101917 / NGR234)
Q02929 1.92e-35 142 33 2 304 3 Cthe_0039 Putative sensory transducer protein Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)
P55652 4.85e-29 125 33 1 281 3 NGR_a01640 Probable chemoreceptor y4sI Sinorhizobium fredii (strain NBRC 101917 / NGR234)
Q88KP1 1.89e-26 116 28 7 362 1 mcpA Methyl-accepting chemotaxis protein McpA Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Q9HW93 7.48e-26 114 28 11 363 1 pctC Methyl-accepting chemotaxis protein PctC Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q52877 1.2e-25 113 31 4 269 3 mcpE Probable chemoreceptor McpE Rhizobium meliloti (strain 1021)
P39216 1.67e-25 114 26 7 385 3 tlpA Methyl-accepting chemotaxis protein TlpA Bacillus subtilis (strain 168)
Q88N45 3.23e-25 112 28 6 339 1 mcpG Methyl-accepting chemotaxis protein McpG Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
P39217 4.98e-25 112 28 6 345 3 tlpB Methyl-accepting chemotaxis protein TlpB Bacillus subtilis (strain 168)
Q88D09 9.52e-25 111 28 3 316 1 mcpQ Methyl-accepting chemotaxis protein McpQ Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
G3XDA3 1.03e-24 111 28 7 338 1 ctpH Methyl-accepting chemotaxis protein CtpH Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q882Z2 1.06e-24 111 30 8 321 3 pscA Methyl-accepting chemotaxis protein PscA Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Q9HW91 6.48e-24 108 28 11 367 1 pctB Methyl-accepting chemotaxis protein PctB Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
P39209 8.26e-24 108 26 4 330 3 tlpC Methyl-accepting chemotaxis protein TlpC Bacillus subtilis (strain 168)
G3XD24 1.43e-23 107 29 9 325 1 pctA Methyl-accepting chemotaxis protein PctA Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q88E10 2.2e-23 107 29 4 323 1 mcpS Methyl-accepting chemotaxis protein McpS Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Q9HUB1 4.83e-23 106 27 7 350 1 mcpK Methyl-accepting chemotaxis protein McpK Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q6HNQ4 9.99e-23 105 26 6 347 3 BT9727_0469 Probable methyl-accepting chemotaxis protein BT9727_0469 Bacillus thuringiensis subsp. konkukian (strain 97-27)
A5W2C8 1.6e-22 104 30 5 243 2 pcaY Methyl-accepting chemotaxis protein PcaY Pseudomonas putida (strain ATCC 700007 / DSM 6899 / JCM 31910 / BCRC 17059 / LMG 24140 / F1)
Q88JK6 1.95e-22 103 30 5 243 1 pcaY Methyl-accepting chemotaxis protein PcaY Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
C0SP89 3.12e-22 103 27 11 392 2 yoaH Putative methyl-accepting chemotaxis protein YoaH Bacillus subtilis (strain 168)
Q9X1E2 5.53e-22 102 29 7 333 1 mcp4 Methyl-accepting chemotaxis protein 4 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
Q9X0M7 3.79e-21 100 29 6 302 1 mcp2 Methyl-accepting chemotaxis protein 2 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
B0R9Z1 3.95e-21 99 33 3 218 1 car Transducer protein Car Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
O32239 4.27e-21 100 24 11 410 3 yvaQ Putative sensory transducer protein YvaQ Bacillus subtilis (strain 168)
Q9R9U8 4.35e-21 99 27 10 342 3 alkN Putative methyl-accepting chemotaxis AlkN Pseudomonas oleovorans
P39215 2.08e-20 98 28 8 339 1 mcpB Methyl-accepting chemotaxis protein McpB Bacillus subtilis (strain 168)
P0C6D8 3.32e-20 97 36 3 172 3 tcpI Toxin coregulated pilus biosynthesis protein I Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
A5F389 3.47e-20 97 36 3 172 3 tcpI Toxin coregulated pilus biosynthesis protein I Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
P39214 5.13e-20 97 28 9 369 1 mcpA Methyl-accepting chemotaxis protein McpA Bacillus subtilis (strain 168)
Q88R14 6.82e-20 96 26 2 313 1 mcpH Methyl-accepting chemotaxis protein McpH Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
P43500 1.8e-19 94 25 2 320 1 frzCD Frizzy aggregation protein FrzCD Myxococcus xanthus
Q0VTI9 2.31e-19 95 33 1 183 3 ABO_0106 Putative methyl-accepting chemotaxis AlkN Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)
Q9I0I4 6.84e-19 93 29 8 323 1 tlpQ Methyl-accepting chemotaxis protein TlpQ Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q9I3S1 1.04e-18 91 38 4 168 1 bdlA Biofilm dispersion protein BdlA Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q9X0N0 2.45e-18 91 32 2 182 1 mcp3 Methyl-accepting chemotaxis protein 3 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
Q9I0I6 5.43e-18 90 28 8 326 1 ctpM Methyl-accepting chemotaxis protein CtpM Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q88NI1 8.84e-18 90 28 9 311 1 mcpU Methyl-accepting chemotaxis protein McpU Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Q88IY8 1.08e-17 89 33 4 203 1 mcpP Methyl-accepting chemotaxis protein McpP Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
P35841 4.94e-17 87 34 6 226 3 dcrA Chemoreceptor protein A Nitratidesulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / CCUG 34227 / NCIMB 8303 / VKM B-1760 / Hildenborough)
Q9I3F6 5.79e-17 87 30 3 225 1 aer Methyl-accepting chemotaxis protein Aer Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
P42257 6e-17 87 26 2 257 1 pilJ Protein PilJ Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q9HPR6 6.12e-17 87 31 4 220 3 hemAT Heme-based aerotactic transducer HemAT Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
P15492 1.57e-16 85 43 2 122 3 hlyB Methyl-accepting chemotaxis protein HlyB Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Q6HP15 1.67e-16 85 26 9 326 3 BT9727_0355 Probable methyl-accepting chemotaxis protein BT9727_0355 Bacillus thuringiensis subsp. konkukian (strain 97-27)
Q9WYR0 3.64e-16 85 27 11 305 1 mcp1 Methyl-accepting chemotaxis protein 1 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
P54576 9.45e-16 84 37 9 193 1 mcpC Methyl-accepting chemotaxis protein McpC Bacillus subtilis (strain 168)
B0R461 4.3e-15 82 29 9 292 1 htr6 Transducer protein Htr6 Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Q55445 6.51e-15 81 37 1 130 3 sll0041 Putative methyl-accepting chemotaxis protein sll0041 Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)
Q9I055 8.1e-15 80 28 9 287 1 mcpN Methyl-accepting chemotaxis protein McpN Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q2W4T8 1.4e-14 80 37 2 130 1 amb2333 Methyl-accepting chemotaxis protein Amb2333 Paramagnetospirillum magneticum (strain ATCC 700264 / AMB-1)
Q2W8M7 2.16e-14 79 33 4 183 1 amb0994 Methyl-accepting chemotaxis protein Amb0994 Paramagnetospirillum magneticum (strain ATCC 700264 / AMB-1)
Q9Z429 2.32e-14 79 26 3 330 3 nahY Methyl-accepting chemotaxis protein NahY Pseudomonas putida
Q9HP81 3.37e-14 79 27 6 284 3 htr2 Sensory rhodopsin II transducer Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
B0R6B1 3.37e-14 79 27 6 284 1 htr2 Sensory rhodopsin II transducer Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
O06477 5.69e-14 75 37 1 125 2 yfmS Putative sensory transducer protein YfmS Bacillus subtilis (strain 168)
Q9HP84 7.49e-14 78 26 10 307 3 cosT Transducer protein CosT Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
B0R6A7 7.49e-14 78 26 10 307 1 cosT Transducer protein CosT Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Q9HUW6 1.09e-13 77 69 0 55 1 ctpL Methyl-accepting chemotaxis protein CtpL Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q9HQ00 9.36e-13 73 36 0 136 3 htr9 Halobacterial transducer protein 9 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
B0R474 1.04e-12 74 28 8 265 1 htr18 Transducer protein Htr18 Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
P0DMI3 4.91e-12 72 27 8 309 1 htr1 Sensory rhodopsin I transducer Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
B0R632 4.91e-12 72 27 8 309 1 htr1 Sensory rhodopsin I transducer Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
B0R6I4 6.77e-12 71 30 9 249 1 basT Transducer protein BasT Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
P42259 1.07e-11 70 35 1 127 1 htr2 Sensory rhodopsin II transducer Natronomonas pharaonis
B0R5T0 1.53e-11 70 24 7 294 1 htr8 Transducer protein Htr8 Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Q9I6V2 2.47e-11 68 46 4 99 1 mcpA Methyl-accepting chemotaxis protein McpA Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q5V5V4 2.77e-11 69 30 1 193 2 htr2 Sensory rhodopsin II transducer Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)
B0R4N9 3.78e-11 68 29 8 232 1 htr13 Transducer protein Htr13 Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
P0DMI4 1.5e-10 67 33 4 178 3 htr4 Transducer protein Htr4 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
B0R470 1.5e-10 67 33 4 178 1 htr4 Transducer protein Htr4 Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
P42258 2.63e-10 66 34 1 149 3 htrII Sensory rhodopsin II transducer (Fragment) Haloarcula vallismortis
Q5UXM8 2.71e-10 66 35 3 134 1 htr1 Sensory rhodopsin I transducer Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)
O07621 3.95e-10 65 34 5 140 1 hemAT Heme-based aerotactic transducer HemAT Bacillus subtilis (strain 168)
B0R367 2.26e-09 63 31 4 163 1 mpcT Transducer protein MpcT Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
P0DMI5 1.9e-08 60 29 5 231 3 htr7 Transducer protein Htr7 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
B0R6A6 1.9e-08 60 29 5 231 1 htr7 Transducer protein Htr7 Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)

  • Number of RefSeq hits:

General

Source Proteus mirabilis HI4320
Locus tag PMI_RS11775
Feature type CDS
Gene -
Product methyl-accepting chemotaxis protein
Location 2603326 - 2604846 (strand: -1)
Length 1521 (nucleotides) / 506 (amino acids)

Contig

Accession NC_010554
Length 4063606 nucleotides
Topology circular
Plasmid False

Orthology

Orthogroup group_2454
Orthogroup size 7
N. genomes 1

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Genomic region

Domains

PF00015 Methyl-accepting chemotaxis protein (MCP) signalling domain
PF00672 HAMP domain

COG entry Annotation(s)

ID Function(s) descr. Function(s) cat. Description
COG0840 Signal transduction mechanisms (T) T Methyl-accepting chemotaxis protein (MCP)

Kegg Ortholog Annotation(s)

KO Description Pathways Modules
K03406 methyl-accepting chemotaxis protein Two-component system
Bacterial chemotaxis		 -

Protein Sequence

MSIERTAKVGSIILLAIFLVTSALSSFFIYQMQDNFASLNSLTTRLNNVQEARYELATIRSHVNFLVNIGHENLDMQETKARLVSEAKTLAAKSKETINAWERESKISADAQRNSEQLSALFIKLVDTIIAPIDTLAYENIDLSKDFSKLAELFDEYLVITQNVNDQIEDEQKWMFQLFIYTTCIALVILIGLLYLVIRWVNKTFLFNLNTLSAILQKVGEGNLNFSLPKNRKDEFGTLFSHVGDMQNALTSTIGLVKEEALVIKSGSAEIAAGNQDLSSRTEEQASALQQTAASMEEIKIAVVNNTNNAVLANSITAQTRDLAIDGASIMNDAITSMKKIEVGTLKVAEINDVVNNIASQTNILALNAAVEAARAGEQGRGFTVVATEVRNLAARSAEAAREINQIIRASVADVAHGRELVNKTGEHIQEIVTSITKVSDIMQGISIASEEQKVGIEQIAVAINQMDSVVQQNAALVEQGATSTMMLDEKAQNLIDKVSVFQIKE

Flanking regions ( +/- flanking 50bp)

CCGATATATGAATACATTTATGTTATTTAATTACCGGGGTGGATTATTTCATGAGTATTGAAAGAACAGCGAAAGTAGGCTCTATTATACTATTAGCTATTTTCTTAGTGACATCAGCTTTATCATCGTTTTTTATTTATCAAATGCAGGATAATTTTGCTTCATTAAACTCGTTAACCACGAGATTAAATAATGTACAAGAGGCAAGATATGAACTAGCAACCATACGCTCTCATGTTAACTTTTTAGTCAATATTGGTCATGAAAATTTAGATATGCAAGAAACCAAAGCTCGATTAGTTTCTGAAGCTAAAACATTAGCGGCTAAATCAAAAGAGACAATTAACGCTTGGGAAAGGGAAAGCAAAATCAGTGCTGACGCACAAAGAAACTCAGAACAATTATCGGCACTATTTATCAAACTCGTCGATACTATTATTGCGCCTATTGATACGCTAGCTTATGAAAATATTGATTTATCGAAAGATTTTTCAAAACTTGCTGAGTTGTTTGATGAATACTTAGTGATAACTCAAAATGTTAATGATCAAATTGAAGACGAACAAAAATGGATGTTTCAACTCTTTATTTACACTACATGTATTGCTTTAGTGATACTGATTGGACTGCTTTATTTAGTGATCCGTTGGGTTAATAAAACCTTTCTCTTTAACCTCAATACGCTCTCTGCCATTTTGCAAAAAGTGGGAGAAGGGAATTTAAACTTCTCGTTACCCAAAAATAGAAAAGATGAGTTTGGTACACTATTTTCTCATGTCGGTGATATGCAAAATGCACTGACATCGACTATTGGTTTGGTGAAAGAAGAGGCATTAGTGATCAAAAGCGGTTCAGCAGAAATCGCAGCAGGTAATCAAGATCTCTCTTCTCGTACAGAAGAGCAAGCGAGTGCATTGCAACAAACCGCAGCCAGCATGGAAGAGATAAAAATTGCGGTAGTGAATAATACCAATAATGCCGTATTAGCAAACTCTATCACAGCACAAACGCGTGATTTGGCTATCGATGGCGCTAGCATTATGAATGATGCCATTACTTCGATGAAAAAAATTGAAGTCGGTACGTTAAAAGTCGCTGAAATCAATGACGTGGTAAACAATATTGCCAGTCAAACCAATATTCTTGCGTTGAATGCGGCAGTGGAAGCCGCTCGTGCAGGGGAGCAAGGGCGGGGTTTTACAGTTGTTGCAACAGAGGTTCGCAATTTAGCGGCCAGAAGTGCGGAAGCCGCTCGTGAAATTAATCAGATTATTCGAGCTTCTGTGGCGGATGTCGCACATGGTCGTGAACTGGTTAATAAAACCGGTGAGCATATACAAGAAATTGTCACCTCAATTACCAAAGTAAGCGATATCATGCAGGGAATTAGCATTGCATCGGAAGAGCAAAAAGTGGGAATTGAACAAATAGCCGTCGCGATAAATCAAATGGATTCTGTGGTTCAGCAAAATGCGGCTTTAGTAGAACAAGGAGCCACCTCAACCATGATGTTAGATGAAAAAGCACAAAATCTGATCGATAAAGTATCAGTTTTTCAAATCAAAGAATAAGCTTATCTTTAATTAATACATTAATATTTTAGTAAAAATAAACTCACCTT